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Free, publicly-accessible full text available January 10, 2026
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In terrestrial plants a basal innate immune system, pattern-triggered immunity (PTI), has evolved to limit infection by diverse microbes. The remodeling of actin cytoskeletal arrays is now recognized as a key hallmark event during the rapid host cellular responses to pathogen attack. Several actin binding proteins have been demonstrated to fine tune the dynamics of actin filaments during this process. However, the upstream signals that stimulate actin remodeling during PTI signaling remain poorly characterized. Two second messengers, reactive oxygen species (ROS) and phosphatidic acid (PA), are elevated following pathogen perception or microbe-associated molecular pattern (MAMP) treatment, and the timing of signaling fluxes roughly correlates with actin cytoskeletal rearrangements. Here, we combined genetic analysis, chemical complementation experiments, and quantitative live-cell imaging experiments to test the role of these second messengers in actin remodeling and to order the signaling events during plant immunity. We demonstrated that PHOSPHOLIPASE Dβ (PLDβ) isoforms are necessary to elicit actin accumulation in response to flg22-associated PTI. Further, bacterial growth experiments and MAMP-induced apoplastic ROS production measurements revealed that PLDβ-generated PA acts upstream of ROS signaling to trigger actin remodeling through inhibition of CAPPING PROTEIN (CP) activity. Collectively, our results provide compelling evidence that PLDβ/PA functions upstream of RBOHD-mediated ROS production to elicit actin rearrangements during the innate immune response in Arabidopsis.more » « less
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OpenMP implementations make increasing demands on the kernel. We take the next step and consider bringing OpenMP into the kernel. Our vision is that the entire OpenMP application, run-time system, and a kernel framework is interwoven to become the kernel, allowing the OpenMP implementation to take full advantage of the hardware in a custom manner. We compare and contrast three approaches to achieving this goal. The first, runtime in kernel (RTK), ports the OpenMP runtime to the kernel, allowing any kernel code to use OpenMP pragmas. The second, process in kernel (PIK) adds a specialized process abstraction for running user-level OpenMP code within the kernel. The third, custom compilation for kernel (CCK), compiles OpenMP into a form that leverages the kernel framework without any intermediaries. We describe the design and implementation of these approaches, and evaluate them using NAS and other benchmarks.more » « less
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In Global Navigation Satellite System (GNSS), a spoofing attack consists of forged signals which possibly cause the attacked receivers to deduce a false position, a false clock, or both. In contrast to simplistic spoofing, the induced spoofing captures the victim tracking loops by gradually adjusting it’s parameters, e.g., code phase and power. Then the victims smoothly deviates from the correct position or timing. Therefore, it is more difficult to detect the induced spoofing than the simplistic one. In this paper, by utilizing the dynamic nature of such gradual adjustment process, an induced spoofing detection method is proposed based on the S-curve-bias (SCB). Firstly, SCB in the inducing process is theoretically derived. Then, in order to detect the induced spoofing, a detection metric is defined. After that, a series of experiments using the Texas spoofing test battery (TEXBAT) are performed to demonstrate the effectiveness of the proposed algorithm.more » « less
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Human activities in buildings are connected by various transportation measures. For the emerging Smart and Connected Communities (S&CC), it is possible to synergize the energy management of smart buildings with the vehicle operation/travel information available from transportation infrastructure, e.g. the intelligent transportation systems (ITS). Such information enables the prediction of upcoming building occupancy and upcoming charging load of electrified vehicles. This paper presents a predictive energy management strategy for smart community with a distributed model predictive control framework, in which the upcoming building occupancy and charging load are assumed to be predictable to certain extent based on the ITS information. An illustrative example of smart community is used for simulation study based on a Modelica simulation model, in which a chilled-water plant sustains the ventilation and air conditioning of three buildings, and each building is assumed to host a number of charging stations. Simulation study is performed to validate the proposed strategy.more » « less
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